Antifungal potential of human pathogenic Blastobotrys sp. by Bacillus licheniformis strain S10 identified using 16S rRNA and first time cultivated on Glycerol Asparagine Agar with implications for NCBI database enhancement

Abstract

A soil sample collected from the rhizosphere of Ocimum tenuiflorum (tulsi), Karad, Maharashtra, India, was diluted to 10-2 and spread on Glycerol Asparagine agar. The isolate was catalase positive, able to utilize starch and ferment the sugar. Molecular identification through partial 16S ribosomal RNA gene sequencing confirmed the isolate as Bacillus licheniformis strain S10, with only 95.22% identity with NCBI sequence database suggesting the implications for NCBI database enhancement. Phylogenetic analysis using the Maximum Likelihood method revealed its closest relationship to the strain KX946197.1:370-1248. The strain showed antifungal activity against human pathogenic Blastobotrys sp.