Ocimum tenuiflorum RHIZOSPHERIC Streptomyces rochei MAY INHIBIT GRWOTH OF Microsporum gypseum; A CAUSATIVE AGENT OF HUMAN FUNGAL INFECTIONS

Abstract

Microsporum gypseum is a human pathogenic fungi causing infections leading to the need of 
antifungal therapy. The streptomyces rochei was isolated from the rhizospheric soil of Ocimum 
tenuiflorum and identified by partial 16S rRNA ribosomal gene sequence. It was then positively 
tested against Microsporum gypseum by a dual culture method. The production of antifungal 
compound was performed in a Glycerol Aspargine Broth and the supernatant was used for the 
purification of antifungal agent by Solvent Extraction method. The ingredients of the supernatant 
were identified by a Gas Chromatography/Mass Spectrometry. The purified crude extract was then 
dissolved in Dimethyl Sulphoxide (DMSO) and its activity against Microsporum gypseum was found 
to be positive. The ingredients of compound were partially separated by a Thin Layer 
Chromatography. The bioautography of the separated components on TLC showed possibility of 
inhibition of Microsporum gypseum.