Antibacterial and antifungal activities of Saussurealappa

Abstract

The current study investigated the antibacterial properties of petroleum ether, chloroform, methanol, and water extracts of Saussurealappa by assessing the zone of inhibition and minimum inhibitory concentration (MIC) against several bacterial and fungal stains. The disc diffusion technique was used to assess in-vitro antibacterial and antifungal activity. In addition, MIC was calculated using the broth dilution method. The different solvent extracts demonstrated concentration-dependent antibacterial activity against four gram-positive (Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, and Bacillus cereus) and four gram-negative (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Salmonella typhi) bacteria.The MIC values against Gram-positive bacteria varied from 50 to 500 μg/ml, while Gram-negative bacteria ranged from 100 to 500 μg/ml. The antifungal activity was found to be strong against three distinct fungi. The extract's MIC values against tested fungus stain ranged from 200 to 1000 μg/ml.