METHOD VALIDATION OF RELATED COMPOUNDS IN THE PERAMPANEL BY USING HPLC

Abstract

Simply rapid, precise, sensitive and reproducible High Performance Liquid Chromatography Method has been developed for the quantitative analysis of perampanel and its impurities was achieved on WatersAlliance-e2695, by using Zorbax SB C18(150x4.6mm,3.5μ) column and the mobile phase containing Water & Acetonitrile in the ratio of (1:1) v/v. The flow rate was 0.8 ml/min; detection was carried out by absorption at 220nm using a photodiode array detector at ambient temperature. The number of theoretical plates and tailing factor for perampanel and its impurities were NLT2000 and NMT2 respectively. %Relative standard deviation of peak areas of measurements always ˂2.0. The proposed method was validated according to ICH guidelines. The method was found to be simple, economical, suitable, precise, accurate& robust method for Quantitatively analysis of perampanel and its impurities and study of its stability.