PREVALENCE OF CEPHAMYCIN-RESISTANT IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN AL- MUTHANA HOSPITALS

Abstract

Background and Objectives: Escherichia coli (E. coli) is an important member of Enterobacteriaceae family involved in severe infections. The aim of this study was to isolate and identify Cephamycins resistant Escherichia coli strains that cause urinary tract infections (UTIs), from outpatient in Al-muthana hospitals in Al-muthana province. Materials and Methods: Of the 472 urine sample tested a total of 88 UPEC were isolated and confirmed with culture on MacConkey agar and Eosine Methylene Blue agar (EMB) and biochemical tests . Antibiotic resistance of the isolates was tested using disk diffusion method towards 20 antibiotics used in the current study. The prevalence of Cephamycins resistant and virulence genes were investigated using PCR. Results: The rate of significant bacteriuria in this study was 182 (38.5%), and E. coli strains proportion was 88 (48.35%), while the rest of the other bacterial species formed the 94 (51.65%), The ranges of resistance and average resistance to antibiotics shown by the studied isolates were determined, and the results showed that all isolates were resistant to a minimum of six classes of antibiotics. It was found that 43 isolate of E.coli (48.86%) resistance to Cefoxitin antibiotic , in addition to 6 isolates, at (6.8%) showed moderate resistance, Conclusion: All AmpC β-lactamase-producing E. coli isolates carry AmpC gene of plasmid origin. As well as all the tested E. coli isolates were multidrug drug resistant; The Carbapenemes (imipenem ) were the drug of choice for the treatment of infections caused by AmpC-producing isolates. In conclusion, the results of the PCR examination showed that the EBC enzyme is the most common AmpC beta-lactamase enzyme among the studied isolates.